Phosphorylated 350 kD protein in the nucleus as it is associated with cell transformation |
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| Authors: | Chikako Sato Kimiko Nishizawa Tokiko Nakayama Reiko Hirai Hiromu Nakamura |
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| Institution: | 1. Laboratory of Experimental Radiology, Aichi Cancer Center Institute, Nagoya 464, Japan;2. Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan;1. Cell Signalling Section, Division of Immunology, Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QP, UK;2. Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA;3. Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 2QQ, UK;1. Department of Colorectal Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Art and Science, Xiangyang, Hubei, China;2. Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China;1. Clinical Laboratory, The Rizhao People''s Hospital Affiliated to Jining Medical University, Rizhao, Shandong, China;2. Department of Blood Transfusion, The Rizhao People''s Hospital Affiliated to Jining Medical University, Rizhao, Shandong, China;3. Department of Medical Image, The Rizhao People''s Hospital Affiliated to Jining Medical University, Rizhao, Shandong, China;4. Department of Anesthesiology, The Rizhao People''s Hospital Affiliated to Jining Medical University, Rizhao, Shandong, China;1. The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510630 , Guangdong Province, China;2. Department of Emergency, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China;3. The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000 , Liaoning Province, China;4. Department of General Surgery, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China;1. School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China;2. Key Laboratory of Structure-based Drug Design ＆ Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its location between the nucleus and cytoplasm of normal cells. Persistent intranuclear location of the phosphorylated 350 kD protein was also found throughout the cell cycle in transformed cells, as detected by immunoprecipitation of 32P-phosphorylated 350 kD protein from isolated nuclei and immunofluorescent staining with a monoclonal antibody that recognized phosphorylated site of 350 kD protein. A conditional transformed phenotype induced by a temperature-sensitive (ts) viral oncogene or a transforming growth factor was also associated with the intranuclear presence of the phosphorylated 350 kD protein. Thus the 350 kD protein seems to be a target molecule of protein kinases that are stimulated directly or indirectly by growth factors or by oncogene products in the nucleus, and appears to be a new transformation-related nuclear antigen. |
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