A Bayesian Computational Approach to Explore the Optimal Duration of a Cell Proliferation Assay |
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Authors: | Alexander P Browning Scott W McCue Matthew J Simpson |
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Institution: | 1.School of Mathematical Sciences,Queensland University of Technology (QUT),Brisbane,Australia |
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Abstract: | Cell proliferation assays are routinely used to explore how a low-density monolayer of cells grows with time. For a typical cell line with a doubling time of 12 h (or longer), a standard cell proliferation assay conducted over 24 h provides excellent information about the low-density exponential growth rate, but limited information about crowding effects that occur at higher densities. To explore how we can best detect and quantify crowding effects, we present a suite of in silico proliferation assays where cells proliferate according to a generalised logistic growth model. Using approximate Bayesian computation we show that data from a standard cell proliferation assay cannot reliably distinguish between classical logistic growth and more general non-logistic growth models. We then explore, and quantify, the trade-off between increasing the duration of the experiment and the associated decrease in uncertainty in the crowding mechanism. |
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