宫颈癌患者人乳头瘤病毒感染情况及STING、ZEB1蛋白水平分析

分析宫颈癌患者人乳头瘤病毒（HPV）感染情况及癌灶组织内干扰素基因刺激因子（STING）、E盒锌指结合蛋白1（ZEB1）表达情况，为该类患者的治疗提供参考。

收集我院病理检验科2019年1月至2022年1月63例宫颈癌标本（宫颈癌组）、55例宫颈上皮内瘤变（CIN）标本（CIN组）及50例正常宫颈标本（正常组），采用免疫组化法检测各标本内STING及ZEB1表达情况，采用原位杂交法检测各标本内高危HPV感染情况，分析STING及ZEB1蛋白表达情况与宫颈癌病理特征及高危HPV感染之间的关系。

宫颈癌组标本STING及ZEB1表达量均高于CIN组及正常组，CIN组标本ZEB1表达量高于正常组，差异均有统计学意义（均P＜0.05）；CIN组标本STING表达量与正常组比较差异无统计学意义（P＞0.05）。宫颈癌组标本HPV16、18检出率高于CIN组及正常组，CIN组标本HPV16、18检出率高于正常组，差异均有统计学意义（均P＜0.05）。宫颈癌患者FIGO分期及浸润深度与其组织内STING及ZEB1蛋白阳性情况均存在相关性；此外，宫颈癌淋巴结转移及肿瘤分化程度与组织内STING蛋白阳性情况存在相关性，差异均有统计学意义（均P＜0.05）。宫颈癌患者STING及ZEB1蛋白表达与其HPV16、18检出率间均呈正相关（均P＜0.05）。

宫颈癌组标本STING及ZEB1蛋白表达量均高于正常组及CIN组，且其表达量还与宫颈癌浸润、FIGO分期相关。STING和ZEB1可能与HPV16、18感染共同参与宫颈癌的发生及进展。

Human papillomavirus infection and levels of STING and ZEB1 proteins in patients with cervical cancer

ObjectiveTo analyze human papillomavirus (HPV) infection and the levels of stimulator of interferon genes (STING) and zinc-finger E-box binding protein 1 (ZEB1) in patients with cervical cancer (CC), and provide a reference for the treatment of thses patients. MethodsSpecimens from 63 cases of CC (CC group), 55 cases of cervical intraepithelial neoplasia (CIN group) and 50 individuals with normal cervix (control group) were collected from the pathological examination department of our hospital from January 2019 to January 2022. Immunohistochemistry was used to detect the expression of STING and ZEB1 in each specimen, and in situ hybridization was performed to detect high-risk HPV infection in each specimen. The relationship of STING and ZEB1 protein expression with the pathological features of CC and high-risk HPV infection was discussed. ResultsThe expression of STING and ZEB1 in CC group was higher than that in CIN group and control group, and the expression of ZEB1 in CIN group was higher than that in control group, the differences were statistically significant (all P＜0.05), while the expression of STING in CIN group showed no statistical difference with that in control group (P＞0.05). The detection rate of HPV16 and HPV18 in CC group were higher than that in CIN group and control group, and the detection rate of HPV16 and HPV18 in CIN group were higher than that in control group, the differences were statistically significant (all P＜0.05). The FIGO stage and invasion depth of CC patients were correlated with the positive expression of STING and ZEB1 in tissues. In addition, lymph node metastasis and tumor differentiation of CC patients were correlated with the positive expression of STING protein in tissues, the differences were statistically significant (all P＜0.05). Correlation analysis showed that the expressionof STING and ZEB1 were positively correlated with the detection rates of HPV16 and HPV18 (all P＜0.05). ConclusionThe expression of STING and ZEB1 is elevated in CC tissues compared with normal cervix and CIN lesions, and their positive expression has correlation with CC infiltration and FIGO stage. STING and ZEB1 together with HPV16 and HPV18 infection may be involved in the development and progression of CC.