Whole amount in situ hybridization and transgene via microinjection in zebrafish] |
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Authors: | Xue Yan Yang Ji Hua Yao Lu Cheng Dong Wang Wei Da Ru Lu Jing Lun Xue |
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Affiliation: | State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China. |
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Abstract: | Zebrafish is new model organism to study development of the vertebrate. In this report, we chose a fragment of gene GATA-1 which specifically expressed in zebrafish hematopoiesis system as probe to carry on whole amount in situ hybridization. Then we transported reporter gene driven by GATA-1 promoter into the embryos via microinjection approach and observed the spatial and temporal expression pattern of GFP. Our results demonstrated that efficient and reliable technology of whole amount in situ hybridization and microinjection in zebrafish was established. |
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