Inactivation of photosynthetic oxygen evolution by o-phenanthroline and LiClO4 in Photosystem 2 of the pea |
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Authors: | Suleyman I Allakhverdiev Hidenori Hayashi Yoko Fujimura Vyacheslav V Klimov Norio Murata |
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Institution: | (1) National Institute for Basic Biology, Myodaiji, 444 Okazaki, Japan;(2) Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia |
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Abstract: | We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30–40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.Abbreviations Chl
chlorophyll
- DCPIP
2,6-dichlorophenol indophenol
- DPC
1,5-diphenylcarbo hydrazide
- EDTA
ethylenediaminetetraacetic acid
- Hepes
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- Mes
4-morpholineethanesulfonic acid
- PBQ
phenyl-p-benzoquinone
- PS 2
Photosystem 2 |
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Keywords: | ,Photosystem 2,oxygen evolution,Mn cluster,
o-phenanthroline,LiClO4
,electron transport |
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