响应面优化超声辅助法提取拐枣种子中二氢杨梅素的工艺

二氢杨梅素和杨梅素是拐枣种子中的重要成分。通过超声辅助法从拐枣种子中提取二氢杨梅素,采用单因素法考察乙醇体积分数、超声辐照功率、提取温度、液料比和超声辐照时间影响参数的基础上,并选用Box-Behnken响应面设计法分析建立了超声辐照功率、超声辐照时间和液料比的二次多项式模型,优化提取工艺。结果得到超声辅助法提取拐枣种子中二氢杨梅素的最佳工艺参数为:乙醇体积分数为60%、超声辐照功率140 W、超声辐照时间30 min、液料比20.5 mL·g-1,提取温度40℃。在此最佳条件下,二氢杨梅素得率为2.14±0.09 mg·g-1。本提取方法简单快速,效率高,有利于拐枣资源的综合加工利用。

Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology

Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb. The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction, ultrasound irradiation power, extraction temperature, liquid-solid ratio and ultrasound irradiation time. The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power, ultrasound irradiation time and liquid-solid ratio, and optimized extraction process. The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%, ultrasonic irradiation power of 140 W, ultrasonic irradiation time of 30 min, liquid-solid ratio of 20.5 mL·g^-1, and extraction temperature of 40℃. Under these optimal conditions, the yield of dihydromyricetin was 2.14±0.09 mg·g^-1. The extraction method is simple, rapid, and high in efficiency, which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.