白桦 BpbHLH112 基因克隆及其启动子表达特性分析

克隆白桦BpbHLH112基因的编码序列,并进行生物信息学分析。通过实时荧光定量PCR技术分析该基因在不同胁迫处理下的表达模式,进一步克隆了BpbHLH112基因上游1446 bp的启动子区域,序列分析表明,该区域含有多个逆境响应、激素响应以及信号转导等相关元件,通过构建BpbHLH112启动子片段融合GUS报告基因的表达载体并进行烟草瞬时转化,分析在不同胁迫处理条件下BpbHLH112启动子驱动GUS基因表达的活性,结果表明BpbHLH112启动子的表达能够被一些逆境胁迫因子诱导,本研究结果为深入研究BpbHLH112调控白桦应对非生物胁迫作用的分子机理提供了理论依据。

Isolation of the BpbHLH112 Gene and Expression Analysis of Its Promoter in Betula platyphylla

The Open Reading Frame sequence of the BpbHLH112 was cloned from Betula platyphylla, and its expression patterns under diverse abiotic stresses were explored. Furthermore, the 1 446 bp promoter region of BpbHLH112 was cloned, and bioinformatics analysis revealed that it harbors numerous cis-regulatory elements associated with signaling molecules and abiotic stress as well as signal transduction. The BpbHLH112 promoter was then analyzed for its ability to control the expression of the GUS reporter gene in transgenic tobacco plants. BpbHLH112-GUS expression was strongly induced by the plant stress regulators in the transformed tobacco plants. These results might provide an important contribution for elucidating the regulation mechanisms of BpbHLH112 expression in responding to the plant stress regulators.