The influence of plectin deficiency on stability of cytokeratin18 in hepatocellular carcinoma |
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Authors: | Chiung-Chi Cheng Yi-Hsiang Liu Chin-Chin Ho Wei-Ting Chao Ren-Jeng Pei Yung-Hsiang Hsu Kun-Tu Yeh Lu-Chang Ho Ming-Chuang Tsai Yih-Shyong Lai |
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Affiliation: | (1) Institute of Medicine of Chung Shan Medical University, Taichung, Taiwan;(2) Department of pathology, Jen Ai Hospital, Taichung, Taiwan;(3) Department of Pathology, Tzu Chi Hospital and University, Hualien, Taiwan;(4) Department of Pathology, Hospital and Medical College of Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, 408, Taiwan;(5) Department of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan;(6) Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77054, USA;(7) Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan;(8) Department of Surgery, Hospital and Medical College of Chung Shan Medical University, Taichung, Taiwan |
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Abstract: | Intermediate filaments are important in building the cellular architecture. Previously we found cytokeratin18 was modulated in human hepatocellular carcinoma. Plectin is a cross-linking protein that organizes the cytoskeleton into a stable meshwork, which can maintain the uniform size and shape of hepatocytes. Because the cells of hepatocellular carcinoma were morphologically different from the hepatocytes, we speculated that expression of plectin and organization of intermediate filament might play roles in the pleomorphism of hepatocellular carcinoma cells. In this paper, we studied the plectin expression of hepatocellular carcinoma and liver tissues by immunohistochemistry and immunoblot. The results revealed that plectin was deficient and cytokeratin18 was modulated in hepatocellular carcinoma. Furthermore, we knockdown the plectin mRNA in Chang cells, the result revealed the plectin was deficient and the organization of cytokeratin18 was altered. Conclusively, this study offers a hypothesis that plectin deficient might play an important role in the tumorigenesis of hepatocellular carcinoma. Dr. Yi-Hsiang Liu contributed equally as the first author. |
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Keywords: | Hepatocellular carcinoma Cytokeratin18 Plectin Stability siRNA |
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