Chitinase Production in Solid-State Fermentation by Enterobacter sp. NRG4 Using Statistical Experimental Design |
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Authors: | Neetu Dahiya Rupinder Tewari Ram Prakash Tiwari Gurinder Singh Hoondal |
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Institution: | (1) Department of Biotechnology, Panjab University, Chandigarh, 160014, India;(2) Department of Microbiology, Panjab University, Chandigarh, 160014, India |
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Abstract: | The optimization of nutrient levels for chitinase production by Enterobacter sp. NRG4 in solid-state fermentation conditions (SSF) was carried out using response surface methodology (RSM) based on central
composite design (CCD). The design was employed by selecting wheat bran-to-flake chitin ratio, moisture level, inoculum size,
and incubation time as model factors. The results of first-order factorial design experiments showed that all four independent
variables have significant effects on chitinase production. The optimum concentrations for chitinase production were wheat
bran-to-flake chitin ratio, 1; moisture level, 80%; inoculum size, 2.6 mL; and incubation time, 168 h. Using this statistical
optimization method, chitinase production was found to increase from 616 U · g−1 dry weight of solid substrate to 1475 U · g−1 dry weight of solid substrate. |
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