In vitro maturation of caprine oocytes in different culture media |
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| Institution: | 1. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran;1. Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles – LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil;2. Institute of Health Sciences, University of International Integration Lusophone African-Brazilian, Acarape, CE, Brazil;3. Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil;4. Laboratory of Reproduction Biotechnology (Biotech), Campus Uruguaiana, Federal University of Pampa, Uruguaiana, Brazil;1. Faculty of Veterinary Medicine, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium;2. Faculty of Medicine and Health Sciences, Department of Basic Medical Sciences - Physiology Group, Ghent University, Ghent, Belgium;1. Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. School of Mechanical Engineering, University of Birmingham, UK;1. Laboratory of Animal Reproduction, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile;2. Laboratory of Animal Physiology, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile |
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| Abstract: | Oocytes were aspirated from caprine ovaries, washed and cultured in TCM-199 containing penicillin, streptomycin and 10, 15, or 20% of fetal bovine serum (FBS), estrous sheep serum (ESS) or estrous goat serum (EGS). After 24–26 h culture, oocytes were separated from cumulus and corona cells by hyaluronidase and by passing through a fine pipette, fixed in acetic alcohol, stained with aceto orcein and observed under a phase-contrast microscope for evidence of maturation. High maturation rates (74–94%) were observed in all concentrations of the three different sera examined. No significant difference was observed between different concentrations and among different sera. Almost no maturation (4%) was observed in the medium lacking protein supplement. In conclusion, these sera with the concentrations examined can be substituted for one another for in vitro maturation of caprine oocytes. |
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