Multi-criteria comparison of resting cell activities of bacterial strains selected for biodesulfurization of petroleum compounds |
| |
| Affiliation: | 1. Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States;2. NMR Facility, Department of Chemistry, Texas A&M, College Station, TX 77843, United States;3. PAMS Facility, Department of Chemistry, University of Georgia, Athens, GA 30602, United States;1. Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Chennai 600 025, India;2. Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore;3. Department of Bioinformatics, Alagappa University, Karaikudi 630 004, India;2. University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington Campus, Leicestershire, LE12 5RD, United Kingdom |
| |
| Abstract: | Five isolates able to utilize dibenzothiophene (DBT) as a sole sulfur source and to convert it to 2-hydroxybiphenyl (HBP) with high rates were selected to investigate their potentialities as biocatalysts of a diesel oil biodesulfurization process. Conventional and chemotaxonomic analyses and 16S ribosomal DNA (rDNA) sequencing showed that these strains belonged to the Rhodococcus/Gordonia cluster. The desulfurizing activities of resting cells were compared under various conditions to evaluate their stability in both aqueous and organic media, their sensitivity to the presence of hexadecane and their sulfur substrate selectivity. In spite of their taxonomic similarity, the five strains exhibited different properties. This diversity was not confirmed by the analysis of the desulfurizing genes by amplification and sequencing of large fragments of dszA, dszB, dszC, and dszD genes which revealed that four of the five selected strains had a dsz genotype identical to those of the reference strain, Rhodococcus erythropolis IGTS8. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
