ESR study of a biological assay on whole blood: antioxidant efficiency of various vitamins |
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| Affiliation: | 1. Institut Méditerranée de Recherche en Nutrition, Service 332, Centre de St-jérôme, 13397 Marseille cédex 20, France;2. Laboratoire Structure et Réactivité des Espèces Paramagnétiques, Université de Provence, centre de St-Jérôme, 13397 Marseille cédex 20, France;3. Spiral 3 rue des Mardors 21560 Couternon, France;1. Department of Physics, Boston University, Boston, MA 02215, USA;2. Division of Materials Science and Engineering, Boston University, Boston, MA 02215, USA;3. Department of Chemistry, Boston University, Boston, MA 02215, USA;4. Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong;1. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;2. Inflammation Research Group, University of Otago, Christchurch, New Zealand;1. Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic;2. Masaryk Memorial Cancer Institute in Brno, Zluty kopec 7, Brno, Czech Republic;3. Karel English College in Brno, Sujanovo namesti 356/1, 602 00 Brno, Czech Republic;1. Free University of Bozen-Bolzano, Faculty of Science and Technology, piazza Università 1, 39100 Bolzano, Italy;2. Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all’Adige, Italy;3. PerfectWine s.r.l., Via della Pieve 70, 37029 S. Floriano, Italy;1. Department of Organic Chemistry, Omsk F.M. Dostoevsky State University, 55a Mira Ave, 644077 Omsk, Russia;2. N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, 9 Lavrentiev Ave, 630090 Novosibirsk, Russia;3. Novosibirsk State University, 2 Pirogova St., 630090 Novosibirsk, Russia;4. Laboratory of New Organic Materials, Omsk State Technical University, 11 Mira Ave, 644050 Omsk, Russia;1. Department of Chemistry-Biochemistry and Physics, University of Québec at Trois-Rivières, C. P. 500, TR, Quebec, Canada G9A 5H7;2. Department of Physics and Atmospheric Science, Sir James Dunn Building Dalhousie University, Lord Dalhousie Drive, Halifax, Canada NS B3H 4R2 |
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| Abstract: | This study deals with the activity of various vitamins against the radical-mediated oxidative damage in human whole blood. We have used a biological method that allows both the evaluation of plasma and that of red blood cell resistance against the free radicals induced by 2,2′-azobis (2-amidinopropane) hydrochloride (AAPH). Spin trapping measures using mainly 5-(diethoxyphosphoryl)-5-methyl-1-pyrolline N-oxide nitrone (DEPMPO) were carried out under several conditions to identify the free radicals implicated in this test. Only the oxygenated-centred radical generated from AAPH was found highly reactive to initiate red blood cell lysis. With DEPMPO only alkoxyl radicals were observed and no evidence was found for alkylperoxyl radicals. The antioxidant activity of several lipid- and water-soluble vitamins has been assessed by the biological assay and through two chemical methods. We have noticed high antioxidant activities for tocopherols (in the order δ>γ>α) in the biological test but not through chemical methods. At 1 μM, the δ-tocopherol efficiency in inhibiting radical-induced red blood cell hemolysis was three times as high as the α-tocopherol efficiency. For β-carotene no significant activity even in whole blood was shown. Highly surprising antioxidant activities were observed for acid folic and pyridoxine, compared to ascorbic acid. At 10 μM, the effectiveness of folic acid was almost three times as high as vitamin C. The biological test seems clinically more relevant than most other common assays because it can detect several classes of antioxidants. |
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