Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition |
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Authors: | Sudhir K Upadhyay Devendra P Singh Ratul Saikia |
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Institution: | (1) Department of Environmental Sciences, B.B. Ambedkar University, Vidya Vihar, Rai Bareilly Road, Lucknow, Uttar Pradesh, 226 025, India;(2) Biotechnology Division, North-East Institute of Science and Technology (CSIR), Jorhat, 785006, Assam, India |
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Abstract: | In this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for
plant growth promoting (PGP) traits at higher salt (NaCl) concentrations (2, 4, 6, and 8%). The results revealed that 24 rhizobacterial
isolates were tolerant at 8% NaCl. Although all the 24 salt tolerable isolates produced indole-3-acetic acid (IAA), while
10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. However, only three isolates
showed the production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Diversity was analyzed through 16S rDNA-RFLP,
and of these isolates with three tetra cutter restriction enzymes (HaeIII, AluI, and MspI), the representative cluster groups were identified by 16S rDNA sequencing. Bacillus and Bacillus-derived genera were dominant which showed PGP attributes at 8% NaCl concentration. Out of 24 isolates, nitrogen fixing ability
(nif H gene) was detected in the two isolates, SU18 (Arthrobacter sp.) and SU48. |
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