Arginase from rat fibrosarcoma. Purification and properties |
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Authors: | R Gopalakrishna B N Agar A Jan |
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Institution: | (1) Department of Microbiology, Cancer Institute, 600 020 Madras |
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Abstract: | Arginase from rat fibrosarcoma was purified about 1900-fold and its properties were compared with those of the enzyme from
liver and kidney. Arginase from fibrosarcoma was a neutral protein of molecular weight 120,000 with a Km value of 11 mM for arginine. The activation energy was 7.2 kcal/mol and the pH optimum was 10. The fibrosarcoma enzyme was
immunologically different from that of the liver. The arginase from fibrosarcoma closely resembled the arginase from the kidney
in its electrophoretic, kinetic and immunological properties. |
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Keywords: | Rat fibrosarcoma arginase immune-precipitation urea cycle |
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