A Parvalbumin-Like Calcium-Binding Protein Is Present in Plant Leaves |
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Authors: | Kajiwara, Hideyuki Okuyama, Tsuneo Fukuda, Minoru Hirano, Hisashi Oono, Kiyoharu |
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Affiliation: | 1National Institute of Agrobiological Resources 2-1-2 Kannondai, Tsukuba, Ibaraki, 305 Japan 2Department of Chemistry, Faculty of Science, Tokyo Metropolitan University Setagaya-ku, Tokyo, 158 Japan |
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Abstract: | A protein with relatively high homology in its N-terminal aminoacid sequence to animal parvalbumin and oncomodulin has beenidentified in leaves of rice (Oryza sativa L.). The PV-likeprotein has a relative molecular mass of 27,000 and an isoelectricpoint of 5.0. This protein was partially purified by ion-exchangechromatography, and the purified protein was found to have Ca2+-bindingactivity in a microscale Ca2+-binding assay. Furthermore, anantiserum raised against a synthetic oligopeptide based on theN-terminal amino acid sequence of this protein cross-reactedwith protein not only from rice but also from other monocotyledonousplants. (Received October 17, 1990; Accepted October 17, 1991) |
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