| 甲醇营养型酵母高密度培养过程中甲醇和乙醇的GC快速检测 |
| |
| 引用本文： | 邓兵兵,方宏清,薛冲,赵洪亮,刘志敏. 甲醇营养型酵母高密度培养过程中甲醇和乙醇的GC快速检测[J]. 工业微生物, 2001, 31(2): 26-29 |
| |
| 作者姓名： | 邓兵兵 方宏清 薛冲 赵洪亮 刘志敏 |
| |
| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
| |
| 基金项目： | 军事医学科学院科技创新研究基金资助项目 |
| |
| 摘 要： | 采用气相色谱法（GC）快速检测甲醇营养型酵母发酵液中的甲醇、乙醇含量，具有样品处理简便，测定时间较短，结果重视性好的特点。在1－10mg/mL范围内具有很好的线性关系。在毕赤酵母高密度表达发酵过程中应用此法对甲醇和乙醇进行实时监空，细胞终密度超过300g/L（干重）。本方法为甲醇营养型酵母工程菌的发酵中试工艺研究提供了重要 的发酵生化参数。
|
| 关 键 词： | 气相色谱 甲醇营养型酵母 甲醇 乙醇 GC 快速检测 高密度培养 表达系统 |
GC rapid analysis of methanol and ethanol during high cell density culture of recombinant methylotrophic yeast |
| |
| DENG Bing-bing,FANG Hong-qing,XUE Chong,ZHAO Hong-liang,LIU Zhi-min. GC rapid analysis of methanol and ethanol during high cell density culture of recombinant methylotrophic yeast[J]. Industrial Microbiology, 2001, 31(2): 26-29 |
| |
| Authors: | DENG Bing-bing FANG Hong-qing XUE Chong ZHAO Hong-liang LIU Zhi-min |
| |
| Abstract: | The concentration of methanol and ethanol during HCDF of yeast was determined by gas chromatographic (GC) procedure. The quantitative repeatibility was available and did not vary with exact time. The analysis time was short and sample procedure was simple. The assay was very linear from 1mg/mL to 10mg/mL. High cell concentrations more than 300g/L dry cell weight in Pichia pastori culture were obtained by the present procedure. The results indicated that it was suitable for rapid analysis of methanol and ethanol during HCDF of reconbinmant methylotrophic yeast. |
| |
| Keywords: | gas chromatography methylotrophic yeast methanol ethanol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
