| 棉花体细胞胚胎发生的研究进展 |
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| 引用本文： | 武秀明,刘传亮,张朝军,李付广.棉花体细胞胚胎发生的研究进展[J].植物学通报,2008,25(4):469-475. |
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| 作者姓名： | 武秀明 刘传亮 张朝军 李付广 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,安阳,455004 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 经过30多年的发展，已经在多个不同的棉花品种中获得了体细胞胚，并再生成苗。但由于体细胞胚胎发生往往受多种因素影响，如何从愈伤组织高效率地转化为胚性愈伤组织依然是限制棉花组织培养与遗传转化的关键问题之一。本文概述了棉花体细胞胚胎发生的研究进展，分别从棉花体细胞胚胎发生的起源、影响棉花体细胞胚胎发生的内外因素以及寻找棉花体细胞胚胎发生特异表达基因等几方面进行综述，并对研究中存在的问题进行了讨论。
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| 关 键 词： | 棉花 影响因素 体细胞胚胎发生 组织培养 |
Progress of Somatic Embryogenesis in Cotton |
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| Xiuming Wu,Chuanliang Liu,Chaojun Zhang,Fuguang Li.Progress of Somatic Embryogenesis in Cotton[J].Chinese Bulletin of Botany,2008,25(4):469-475. |
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| Authors: | Xiuming Wu Chuanliang Liu Chaojun Zhang Fuguang Li |
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| Affiliation: | ( Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455004, China) |
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| Abstract: | Over the past 30 years, somatic embryogenesis induction and plant regeneration has been successful in many different cotton cultivars. However, the efficient conversion of callus into embryogenic callus is still a restricting step during cotton tissue culture and genetic transformation, because embryogenic callus induction is affected by many factors. This paper reviews the research into cotton embryogenic callus in terms of the origin, intrinsic and extrinsic factors affecting callus differentiation, as well as the techniques used to investigate the differential gene expression in the conversion process. We also discuss the problems encountered during the research. |
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| Keywords: | cotton factors somatic embryogenesis tissue culture |
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