Sperm coating mechanism from the 1.8 A crystal structure of PDC-109-phosphorylcholine complex |
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Authors: | Wah David A Fernández-Tornero Carlos Sanz Libia Romero Antonio Calvete Juan J |
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Institution: | Centro de Investigaciones Biológicas, CSIC, Madrid, Spain. |
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Abstract: | Bovine seminal plasma PDC-109 binds to sperm surface choline lipids and promotes sperm capacitation by stimulating the efflux of cholesterol and phospholipids. The structure of PDC-109 with bound phosphorylcholine was solved using MAD data of a single platinum site. Its two globular (40 x 50 x 20 A(3)) Fn2 domains are linked and clustered by a short polypeptide. The choline binding sites lie at the same face of the molecule. Phosphorylcholine binds to the Fn2 domains through a cation-pi interaction between the quaternary ammonium group and a core tryptophan, plus hydrogen bonding between hydroxyls of exposed tyrosines and the phosphate group. The structure of the PDC-109-oPC complex provides a structural ground for the sperm membrane-coating mechanism underlying PDC-109-induced capacitation. |
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