Europium cryptate labeled deoxyuridine-triphosphate analog: synthesis and enzymatic incorporation |
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Authors: | Alpha-Bazin B Bazin H Guillemer S Sauvaigo S Mathis G |
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Institution: | CIS biointernational/DIVT/Research and New technologies, Bagnols/Cèze, France. |
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Abstract: | The synthesis of an europium tris-bipyridine cryptate labeled 2'-deoxyuridine-5 '-triphosphate analog (K-11-dUTP) is described. This labeled triphosphate was incorporated into DNA through enzymatic reactions with terminal transferase and DNA polymerases. The enzymatic reactions were monitored by TRACE (Time Resolved Amplification of Cryptate Emission), a homogeneous method using Fluorescence Resonance Energy Transfer (FRET) from an europium cryptate as donor to a modified allophycocyanine as acceptor. |
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