Deletion/insertion polymorphism of the prion protein gene (<Emphasis Type="Italic">PRNP</Emphasis>) in Polish Holstein-Friesian cattle |
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| Authors: | Urszula Czarnik Tadeusz Zabolewicz Janusz Strychalski Grzegorz Grzybowski Marcin Bogusz Krzysztof Walawski |
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| Institution: | (1) Faculty of Animal Bioengineering, University of Warmia and Mazury, Olsztyn, Poland;(2) Faculty of Biology and Earth Science, University of Nicolaus Copernicus, Torun, Poland; |
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| Abstract: | The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3’ untranslated region (14 bp). DNA was isolated from blood of
234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004.
No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls
in the 3 analysed polymorphic sites within thePRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows. |
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