Inhibition of hexose transport by glucose in a glucose-6-phosphate isomerase mutant ofSaccharomyces cerevisiae |
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| Authors: | A Alonso C Pascual C Romay L Herrera A Kotyk |
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| Institution: | (1) Department of Genetics, National Center for Scientific Research, Havana, Cuba;(2) Department of Clinical Biochemistry, National Center for Scientific Research, Havana, Cuba;(3) Genetic Engineering and Biotechnology Center, Havana, Cuba;(4) Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia |
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| Abstract: | The rate of hexose transport was approximately 60% lower for both the high- and the low-affinity components of hexose uptake
when a glucose-6-phosphate isomerase mutant ofSaccharomyces cerevisiae was preincubated with glucose, as compared with preincubation with water. Similarly theJ
max value of the high-affinity system of the mutant was 25–35 % of the correspondingJ
max value for normal cells incubated with glucose. Accumulation of glucose 6-phosphate or of some other metabolite, such as fructose
6-phosphate or trehalose, may be responsible for this striking inhibition. |
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