Development of a new thiol-reactive prosthetic group for site-specific labeling of biomolecules with radioactive iodine |
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Authors: | Ha Eun Shim Sajid Mushtaq Lee Song Chang Heon Lee Hyosun Lee Jongho Jeon |
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Institution: | 1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea;2. Department of Chemistry, Kyungpook National University, Daegu 41566, Republic of Korea;3. Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology, Daejeon 34113, Republic of Korea |
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Abstract: | In this report, we describe the radiosynthesis of a new thiol-targeting prosthetic group for efficient radioactive iodine labeling of biomolecules. Radioiodination using the precursor 3 was performed to obtain 125I-labeled tetrazole 4b with high radiochemical yield (73%) and radiochemical purity. Using the radiolabeled 4b, a single free cysteine containing peptide and human serum albumin were labeled with 125I in modest-to-good radiochemical yields (65–99%) under mildly reactive conditions. A biodistribution study of 125I]7 in normal ICR mice exhibited lower thyroid uptake values than those of 125I-labeled human serum albumin prepared via a traditional radiolabeling method. Thus, 125I]7 could be employed as an effective radiotracer for molecular imaging and biodistribution studies. The results clearly demonstrate that 4b has the potential to be effectively implemented as a prosthetic group in the preparation of radiolabeled biomolecules. |
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Keywords: | Radiolabeling Radioactive iodine Site-specific reaction Radiopharmaceutical Biodistribution |
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