Regulators of protein metabolism are affected by cyclical nutritional treatments with diets varying in protein and energy content |
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| Authors: | Sourour Boussaid-Om Ezzine Sonia Métayer-Coustard Anne Collin Nicole Rideau Christine Leterrier Isabelle Bouvarel Iban Seiliez Sophie Tesseraud |
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| Affiliation: | 1. Department of Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan;2. Department of Internal Medicine, Haimoto Clinic, 1–80 Yayoi-cho, Kasugai, Aichi 486-0838, Japan;3. Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan;1. Liggins Institute, University of Auckland, Auckland, New Zealand;2. Riddet Institute, Massey University, Palmerston North, New Zealand;3. Food Nutrition & Health, AgResearch Ltd, Palmerston North, New Zealand;4. The High-Value Nutrition National Science Challenge, Auckland, New Zealand;5. Discipline of Nutrition, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand;6. Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand;7. Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand;8. School of Kinesiology, University of British Columbia, Vancouver, British Columbia, Canada;9. Department of Nutrition Exercise and Sports, University of Copenhagen, Copenhagen,Denmark;10. Department of Nutritional Sciences and Research Platform Active Ageing, University of Vienna, Vienna, Austria;11. Department of Human Nutrition, University of Otago, Dunedin, New Zealand;12. Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Sinapore;1. Alliance for Research in Exercise, Nutrition and Activity, Sansom Institute for Health Research, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia;2. Food and Nutrition, Commonwealth Scientific and Industrial Research Organisation, PO Box 10041, Adelaide, SA 5000, Australia;3. Clinical Nutrition Research Centre, School of Biomedical Sciences and Pharmacy, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia;4. School of Psychology, University of Leeds, Leeds LS2 9JT, United Kingdom |
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| Abstract: | There is evidence that the E3 ubiquitin ligases muscle ring finger-1 (MuRF1) and atrogin-1, which mediate the ubiquitination of certain proteins and thereby their proteolysis, are regulated by cyclical nutritional treatments varying in lysine content. In order to explore further the regulatory mechanisms involved in metabolic adaptation to dietary changes, we investigated the effects of daily variations in energy [2800 (E?) followed by 3200 kcal/kg (E+)], protein [230 (P+) followed by 150g/kg (P?)] or both [E?P+ followed by E+P?] on muscle protein metabolism in 2-week-old male broiler chickens. Growth performance was similar for all treatments. Expression of atrogin-1 and MuRF1 was changed by alternation of diets varying in protein (higher expression with P? vs. P+) and energy content (higher expression with E? vs. E+). The expression of atrogin-1 was regulated with mixed diets (increase in E+P? vs. E?P+) but not that of MuRF1. Such regulation may involve the mammalian target of rapamycin (mTOR), which was more phosphorylated with P+ than with P?. Eukaryotic initiation factor 4E binding protein, p70S6 kinase and ribosomal protein S6, which are mTOR targets known to control protein synthesis, were highly activated by increased protein content (P+ vs. P?). The mechanisms coordinating the protein synthesis/proteolysis balance remain to be characterized. |
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