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Genetic engineering of attenuated malaria parasites for vaccination
Authors:Shahid M Khan  Chris J Janse  Stefan HI Kappe  Sebastian A Mikolajczak
Affiliation:1. Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;2. Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA;3. Department of Global Health, University of Washington, Seattle, WA 98195, USA;3. From the Departments of Cell Biology and;4. Departments of Biochemistry, Duke University Medical Center, Durham, North Carolina 27701-3709;1. Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA;2. The National Institutes of Health, National Institute of Allergy and Infectious Diseases, DEA, Rockville, MD 20852, USA;3. Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA 99164-6630, USA;4. Department of Microbiology, Biomedicine Discovery Institute, Monash University, Victoria 3800, Australia;5. Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA;6. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;7. Department of Infectious Diseases and Immunology, Emerging Pathogens Institute University of Florida, Gainesville, FL 32611-0880, USA;1. Department of Research for Health, Science Division, WHO, Geneva, Switzerland;2. School of Public Health, University of Health and Allied Sciences, Ho, Ghana;1. Center for Infectious Disease Research, Formerly Seattle Biomedical Research Institute, 307 Westlake Ave. N, #500, Seattle, WA 98109, United States;2. Department of Global Health, University of Washington, Seattle, WA, United States
Abstract:
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  • Highlights? Genetically attenuated parasites (GAPs) as potential vaccines against malaria. ? Attenuated Plasmodium sporozoites confer sustained sterile immunity against malaria. ? Rodent malaria models are an important in vivo platform to delineate GAP safety and potency. ? Late liver-arresting GAPs elicit superior protective immunity than early arresting parasites. ? P. falciparum GAP vaccine candidates need ultimately to be validated in clinical studies.
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