Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging |
| |
| Authors: | Yoshihiro Nakajima Tomomi Yamazaki Shigeaki Nishii Takako Noguchi Hideto Hoshino Kazuki Niwa Vadim R Viviani Yoshihiro Ohmiya |
| |
| Institution: | 1. National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.; 2. Tsuruga Institute of Biotechnology, TOYOBO Co., Ltd., Tsuruga, Fukui, Japan.; 3. Laboratório de Bioquímica e Biotecnologia de Sistemas Bioluminescentes, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, São Paulo, Brazil.;New Mexico State University, United States of America |
| |
| Abstract: | We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of importin α by fusing ELuc at the intracellular level. These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc. |
| |
| Keywords: | |
|
|
