Single Vector System for Efficient N-myristoylation of Recombinant Proteins in E. coli
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Authors: | Julian M. Glück Silke Hoffmann Bernd W. Koenig Dieter Willbold |
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Affiliation: | 1. Institute of Structural Biology and Biophysics, Research Centre Jülich, Jülich, Germany.; 2. Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.;University of California Davis, United States of America |
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Abstract: | BackgroundN-myristoylation is a crucial covalent modification of numerous eukaryotic and viral proteins that is catalyzed by N-myristoyltransferase (NMT). Prokaryotes are lacking endogeneous NMT activity. Recombinant production of N-myristoylated proteins in E. coli cells can be achieved by coexpression of heterologous NMT with the target protein. In the past, dual plasmid systems were used for this purpose.Methodology/Principal FindingsHere we describe a single vector system for efficient coexpression of substrate and enzyme suitable for production of co- or posttranslationally modified proteins. The approach was validated using the HIV-1 Nef protein as an example. A simple and efficient protocol for production of highly pure and completely N-myristoylated Nef is presented. The yield is about 20 mg myristoylated Nef per liter growth medium.Conclusions/SignificanceThe single vector strategy allows diverse modifications of target proteins recombinantly coexpressed in E. coli with heterologous enzymes. The method is generally applicable and provides large amounts of quantitatively processed target protein that are sufficient for comprehensive biophysical and structural studies. |
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