模拟增温对青海湖鸟岛土壤产甲烷菌群落特征的影响

甲烷生成与产甲烷菌群落关系密切,探究温度升高对产甲烷菌群落的影响可以预测产甲烷菌群落特征对气候变暖的响应.以青海湖鸟岛为研究对象,通过高通量测序方法开展研究.结果表明,基于97％的序列相似度聚类,共得到697个操作分类单元.鸟岛土壤产甲烷菌群落以甲烷微菌目(Methanomicrobiales)、甲烷八叠球菌目(Met...

Effects of Simulated Warming on Methanogens Community Characteristics in Bird Island of Qinghai Lake

Methane production has close relation to the methanogens community, study on the influence of temperature rise on the methanogens community can predict response of the community characteristics of methanogens to climate warming. In this paper, Bird Island in Qinghai Lake is taken as an object, and high-throughput sequencing method is used to carry out the research. The results show that 697 operational taxonomic units are obtained based on 97% sequence similarity clustering. The dominant bacteria in soil methanogenic community of Bird Island are Methanomicrobiales, Methanosarcinales and Methanobacteriales. Temperature increase had no significant effect on the diversity index of soil methanogens community, but had significant effect on the structure of methanogens community. The increased temperature treatment significantly increased the relative abundance of Methanomicrobia, Methanosarcinales, Methermicoccaceae, and Methanotrichaceae, and significantly decreased the relative abundance of Thermoplasmata, Methanomassiliicoccales, and Methanomassiliicoccaceae. FAPROTAX function predicted that methanogens on Bird Island had 15 functional groups, and most methanogens generated methane by H₂/CO₂ nutrition-type pathway. On the whole, as compared with the community diversity of methanogens in soil of Bird Island, the community structure of methanogens was more sensitive to temperature rise, and the relative abundance of some methanogens varied significantly.