鳜肌酸激酶M-CK cDNA的克隆与组织表达分析

利用RT–PCR和cDNA末端快速扩增法（RACE）克隆了鳜（Siniperca chuatsi）肌酸激酶（creatine kinase，CK）cDNA序列，并分析了该基因的结构特征和系统关系。鳜CK cDNA序列全长1586 bp，包括5′端非翻译区92 bp，3′端非翻译区348 bp和开放阅读框（ORF）1 146 bp，共编码381个氨基酸。鳜CK具有脊椎动物CK共有的保守结构域和肌型肌酸激酶（M-CK）同工酶的特异识别位点；氨基酸序列与M-CK型的相似度最高，而与脑型肌酸激酶（B-CK）和线粒体型肌酸激酶（Mi-CKs）的相似度较低；在CK系统关系树中鳜CK与M-CK群聚类。这些均表明，鳜CK属脊椎动物M-CK型。RT-PCR分析表明，鳜M-CK在成体不同组织中的表达量不同，其中，在皮肤、卵巢、肾脏、胃、肌肉和心脏中表达较强；而在眼和脑、肝胰脏中表达较弱。

Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish, Siniperca chuatsi

The creatine kinase(CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods.The structural characteristics and phylogeny of this gene were analyzed.Sequence analysis revealed a 1586 bp cDNA sequence containing 92 bp 5'-untranslated region,348 bp 3'-untranslated region and 1146 bp open reading frame (ORF),which encoded 381 amino acids.Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK(M-CK) isozyme were identif...