Gel electrophoresis studies of proteins from leaves of photoperiodically induced and vegetative cocklebur plants |
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Authors: | SHERWOOD SHARON B; EVANS JOHN O; ROSS CLEON |
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Institution: | Department of Botany and Plant Pathology, Colorado State University Fort Collins, U. S. A. |
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Abstract: | Comparisons of proteins synthesized in photoperiodically-sensitiveleaves of induced and vegetative cocklebur plants were made.Fifteen or more protein bands could be separated by polyacrylamidegel electrophoresis when polyvinylpyrrolidone was used in theextracting buffer to remove phenolics. As contrasted to resultsof others with Pharbilis nil, no differences in stained bandingpatterns could be detected in vegetative and induced plants.Radioactive leucine, lysine and phenylalanine were incorporatedinto similar leaf proteins during the last half of an inductivedark period. These experiments and dual-labeling studies with3H- and 14C-phenylalanine indicated no consistent differencesin the types of extracted proteins synthesized by leaves ofvegetative and induced plants.
1Present address: Plant Science Dept., Utah State University,Logan 84321, U.S.A. (Received October 26, 1970; ) |
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