Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131 |
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Authors: | Heinemann C van Hylckama Vlieg J E Janssen D B Busscher H J van der Mei H C Reid G |
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Institution: | Department of Microbiology and Immunology, University of Western Ontario Health Sciences Centre, London, Ont., Canada. |
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Abstract: | Lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. One strain, Lactobacillus fermentum RC-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. Using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against Enterococcus faecalis 1131 was purified. The N-terminal sequence of the 29-kDa protein was identical to that of a collagen-binding protein from Lactobacillus reuteri NCIB 11951, and exhibited close homology with a basic surface protein from L. fermentum BR11. The results suggest that this anti-adhesive cell surface protein of Lactobacillus could protect against uropathogens by preventing their adhesion. the Federation of European Microbiological Societies. |
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Keywords: | Lactobacillus Enterococcus Bacterial adhesion Collagen |
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