Detection of functional ligand-binding events using synchrotron x-ray scattering |
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Authors: | Rodi Diane J Mandava Suneeta Gore David B Makowski Lee Fischetti Robert F |
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Affiliation: | Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439,USA. drodi@anl.gov |
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Abstract: | Small-molecule ligands that change the structure of a protein are likely to affect its function, whereas those causing no structural change are less likely to be functional. Wide-angle x-ray scattering (WAXS) can be easily carried out on proteins and small molecules in solution in the absence of chemical tags or derivatives. The authors demonstrate that WAXS is a sensitive probe of ligand binding to proteins in solution and can distinguish between nonfunctional and productive binding. Furthermore, similar ligand-binding modes translate into similar scattering patterns. This approach has high potential as a novel, generic, low-throughput assay for functional ligand binding. |
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