Sensitive protein detection via triple-binder proximity ligation assays |
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Authors: | Schallmeiner Edith Oksanen Elli Ericsson Olle Spångberg Lena Eriksson Susann Stenman Ulf-Håkan Pettersson Kim Landegren Ulf |
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Affiliation: | Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Se-75185 Uppsala, Sweden. edith.schallmeiner@genpat.uu.se |
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Abstract: | The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA. |
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