Production of diosgenin by hairy root cultures ofTrigonella foenum-graecum L. |
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Authors: | A Merkli P Christen I Kapetanidis |
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Institution: | (1) Department of Pharmacognosy, University of Geneva, Sciences II, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland |
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Abstract: | Hairy root cultures ofTrigonella foenum-graecum L. were established withAgrobacterium rhizogenes strain A4. The hairy roots produce diosgenin, an important spirostanol for the semi-synthesis of steroid hormones. Fourteen different liquid media were investigated. The fastest growth was obtained in McCown's woody plant (WP) medium supplemented with 3% sucrose; the highest diosgenin content was observed in half-strength WP medium with 1% sucrose (0.040% dry weight), which represents almost twice the amount detected in the 8-month-old non-transformed roots (0.024%). A time-course study in WP liquid media supplemented with 3% sucrose was undertaken. In these conditions, 17 g diosgenin/g fresh weight were produced. The influence of cholesterol, medium pH and chitosan on diosgenin production was tested. The addition of 40 mg/l chitosan elevated the diosgenin content to three times that found in non-elicited hairy roots.Abbreviations
MS
Murashige and Skoog (1962) medium
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WP
McCown's woody plant medium |
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Keywords: | Trigonella foenum-graecum Hairy roots Diosgenin Elicitor |
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