Evidence for programmed cell death and activation of specific caspase-like enzymes in the tomato fruit heat stress response |
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Authors: | Gui-Qin Qu Xiang Liu Ya-Li Zhang Dan Yao Qiu-Min Ma Ming-Yu Yang Wen-Hua Zhu Shi Yu Yun-Bo Luo |
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Institution: | (1) College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, 100083 Beijing, China |
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Abstract: | The tomato (Lycopersicon esculentum) fruit is the best available model to study the stress response of fleshy fruit. Programmed cell death (PCD) plays an important
role in stress responses in mammals and plants. In this study, we provide evidence that PCD is triggered in the tomato fruit
heat stress response by detection of the sequential diagnostic PCD events, including release of cytochrome c, activation of caspase-like proteases and the presence of TUNEL-positive nuclei. Investigating the time course of these events
for 12 h after heat treatment indicated that cytochrome c release and caspase-like protease activation occurred rapidly and were consistent with the onset of DNA fragmentation. In
addition, LEHDase and DEVDase enzymes were specifically activated in tomato fruit pericarp during the heat treatment and recovery
time. There was no significant activation of YVADase or IETDase proteases. Preincubation of pericarp discs with the broad-spectrum,
cell-permeable caspase inhibitor Z-VAD-FMK, suppressed heat-induced cell death measured by trypan blue, accompanied by a decrease
in LEHDase and DEVDase activities.
Gui-Qin Qu and Xiang Liu contributed equally to this work. |
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Keywords: | Caspase-like activity Heat stress Lycopersicon (fruit) Programmed cell death |
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