Hydrolase and fructosyltransferase activities implicated in the accumulation of different chain size fructans in three Asteraceae species. |
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| Authors: | Nair M Itaya Amanda F Asega Maria Angela M Carvalho Rita de Cássia L Figueiredo-Ribeiro |
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| Institution: | 1. Normandie Univ, Caen Cedex, France;2. UCBN, UMR 950 Ecophysiologie Végétale & Agronomie, nutritions NCS, F-14032 Caen, France;3. INRA, UMR 950 EVA, F-14032 Caen, France;4. Station de recherche en agroalimentaire de l’Université du Québec en Abitibi-Témiscamingue, 79, rue Côté, Notre-Dame-du-Nord, QC, Canada J0Z 3B0;1. Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Jalisco (ITTJ), km 10 Carretera a San Miguel Cuyutlán, CP 45640 Tlajomulco de Zúñiga, Jalisco, Mexico;2. Unidad de Biotecnología e Ingeniería Genética de Plantas, Cinvestav-Unidad Irapuato, km 9.6, Libramiento Norte Carretera Irapuato-León, CP 36821 Irapuato, Guanajuato, Mexico;1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China;2. School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China;2. Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom;3. European Molecular Biology Laboratory (EMBL), Hamburg Outstation, Notkestrasse 85, 22603 Hamburg, Germany;1. Department of Biophysics and Biotechnology, Voronezh State University, Universitetskaya sq. 1, Voronezh 394006, Russia;2. Sevastopol State University, Universitetskaya str., 33, Sevastopol 299053, Russia;3. Voronezh State University, Universitetskaya sq. 1, Voronezh 394006, Russia;4. Baltic Federal University, Nevskogo str., 14?A., Kaliningrad 236041, Russia |
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| Abstract: | Fructans are widely distributed in Asteraceae from floras with seasonal growth and are thought to be involved in drought and freezing tolerance, in addition to storage function. Reserve organs of Vernonia herbacea and Viguiera discolor, from the cerrado, and of the perennial herb Smallanthus sonchifolius, endemic to Andean region, store over 80% inulin, with different DP (35, 150, and 15, respectively). The fructan pattern in Asteraceae species could be explained by characteristics of their respective 1-FFTs. Hydrolases and fructosyltransferases from S. sonchifolius, V. herbacea and V. discolor were analyzed in plants at the same environmental conditions. The higher 1-FEH activities found in the species with lower DP, S. sonchifolius and V. herbacea reinforce the hypothesis of the involvement of 1-FEH in fructan profile and suggest that the high DP fructan of V. discolor is a consequence of the low affinity of its 1-FEH to the native long chain inulin. Long term incubation with sucrose suggested that the affinity of 1-FFT of V. discolor for 1-kestose is low when compared to that of V. herbacea. Indeed 1-FFT from V. discolor was shown to be an hDP 1-FFT, preferring longer inulins as acceptors. Conversely, 1-FFT from V. herbacea seems to have a higher affinity for short fructo-oligosaccharides, including 1-kestose, as acceptor substrates. Differences in fructan enzymes of the three Asteraceae provide new information towards the understanding of fructan metabolism and control of carbon flow between low and high DP fructans. |
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