Beta chloroplast genomes: analysis of Fraction I protein and chloroplast DNA variation |
| |
Authors: | Y Kishima T Mikami A Hirai M Sugiura T Kinoshita |
| |
Institution: | (1) Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, 060 Sapporo, Japan;(2) Graduate Division of Biochemical Regulation, Faculty of Agriculture, Nagoya University, Chikusa, 464 Nagoya, Japan;(3) Center for Gene Research, Nagoya University, Chikusa, 464 Nagoya, Japan |
| |
Abstract: | Summary The interrelationships of Beta chloroplast genomes have been investigated on the basis of the analysis of Fraction I protein and chloroplast (ct) DNA. Three groups of the chloroplast genomes could be demonstrated by the difference in isoelectric points of the large subunit of Fraction I protein. Restriction enzyme analysis revealed inter- and intra-specific variations among the ctDNAs, which enabled us to detect seven distinct ctDNA types. In Vulgares and Corollinae species, the observed differences were physically mapped taking advantage of the restriction fragment map available for sugar beet (B. vulgaris) ctDNA. The DNA variations were found to result either from gains or losses of restriction sites or from small deletions/ insertions, and most of them were located in the large single-copy region of the genome. Moreover, the ctDNAs from Patellares species are more diverged from those of other Beta taxa. Our results also indicate that there is a close correlation between the chloroplast genome diversity and the accepted taxonomic classification of the species included in this survey. |
| |
Keywords: | Beta Fraction I protein Chloroplast DNA Comparative restriction site mapping Phylogenetic relationship |
本文献已被 SpringerLink 等数据库收录! |
|