Serum thioredoxin reductase levels increase in response to chemically induced acute liver injury |
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Authors: | Kang Sun,Sofi E. Eriksson,Yanping Tan,Le Zhang,Elias S.J. Arné r,Jinsong Zhang |
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Affiliation: | 1. School of Tea and Food Science, Anhui Agricultural University, Hefei 230036, Anhui, PR China;2. Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden |
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Abstract: | BackgroundMammalian thioredoxin reductases (TrxR) are selenoproteins with important roles in antioxidant defense and redox regulation, principally linked to functions of their main substrates thioredoxins (Trx). All major forms of TrxR are intracellular while levels in serum are typically very low.MethodsSerum TrxR levels were determined with immunoblotting using antibodies against mouse TrxR1 and total enzyme activity measurements were performed, with serum and tissue samples from mouse models of liver injury, as triggered by either thioacetamide (TAA) or carbon tetrachloride (CCl4).ResultsTrxR levels in serum increased upon treatment and correlated closely with those of alanine aminotransferase (ALT), an often used serum biomarker for liver damage. In contrast, Trx1, glutathione reductase, superoxide dismutase or selenium-containing glutathione peroxidase levels in serum displayed much lower increases than TrxR or ALT.ConclusionsSerum TrxR levels are robustly elevated in mouse models of chemically induced liver injury.General significanceThe exaggerated TrxR release to serum upon liver injury may reflect more complex events than a mere passive release of hepatic enzymes to the extracellular milieu. It can also not be disregarded that enzymatically active TrxR in serum could have yet unidentified physiological functions. |
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Keywords: | ALT, alanine aminotransferase CCl4, carbon tetrachloride DTNB, dithio-bis-nitrobenzoic acid GPx, glutathione peroxidase GR, glutathione reductase i.p., intraperitoneally Nrf2, nuclear factor erythroid 2-related factor 2 PBS, phosphate buffer saline SOD, superoxide dismutase TAA, thioacetamide Trx, thioredoxin TrxR, thioredoxin reductase |
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