Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells |
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Authors: | Mariana C.C. Silva,Clá udia A.A. de Paula,Joana G. Ferreira,Edgar J. Paredes-Gamero,Angela M.S.F. Vaz,Misako U. Sampaio,Maria Tereza S. Correia,Maria Luiza V. Oliva |
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Affiliation: | 1. Departamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, Brazil;2. Departamento de Biofísica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, Brazil;3. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão 915, 22460-030 Rio de Janeiro, RJ, Brazil;4. Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego s/n, 50670-910 Recife, PE, Brazil |
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Abstract: | BackgroundPlant lectins have attracted great interest in cancer studies due to their antitumor activities. These proteins or glycoproteins specifically and reversibly bind to different types of carbohydrates or glycoproteins. Breast cancer, which presents altered glycosylation of cell surface glycoproteins, is one of the most frequent malignant diseases in women. In this work, we describe the effect of the lectin Bauhinia forficata lectin (BfL), which was purified from B. forficata Link subsp. forficata seeds, on the MCF7 human breast cancer cellular line, investigating the mechanisms involved in its antiproliferative activity.MethodsMCF7 cells were treated with BfL. Viability and adhesion alterations were evaluated using flow cytometry and western blotting.ResultsBfL inhibited the viability of the MCF7 cell line but was ineffective on MDA-MB-231 and MCF 10A cells. It inhibits MCF7 adhesion on laminin, collagen I and fibronectin, decreases α1, α6 and β1 integrin subunit expression, and increases α5 subunit expression. BfL triggers necrosis and secondary necrosis, with caspase-9 inhibition. It also causes deoxyribonucleic acid (DNA) fragmentation, which leads to cell cycle arrest in the G2/M phase and a decrease in the expression of the regulatory proteins pRb and p21.ConclusionBfL shows selective cytotoxic effect and adhesion inhibition on MCF7 breast cancer cells.General significanceCell death induction and inhibition of cell adhesion may contribute to understanding the action of lectins in breast cancer. |
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Keywords: | MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide BfL, Bauhinia forficata lectin PI, propidium iodide ANOVA, analysis of variance DMEM, Dulbecco's Modified Eagle's Medium DMSO, dimethyl sulfoxide DTT, dithiothreitol FBS, fetal bovine serum FITC, fluorescein isothiocyanate BSA, bovine serum albumin SDS, sodium dodecyl sulfate EGTA, ethylene glycol-bis(2-aminoethylether)-N,N,N&prime ,N&prime -tetraacetic acid HRP, horseradish peroxidase NaF, sodium fluoride |
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