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A DNA sequence cleaved by restriction endonuclease R.EcoRI in only one strand.
Authors:J O Bishop
Affiliation:Department of Genetics University of Edinburgh West Mains Road, Edinburgh EH9 3JN, Scotland
Abstract:Restriction endonuclease EcoRI cuts both strands of the DNA sequence
/></figure> generating two separate frayed ends (Hedgpeth <em>et al.</em>, 1972). Here it is shown that under standard digestion conditions, the enzyme also attacks the sequence <figure class=/></figure> but cuts only one strand. The resulting nick is an efficient initiation point for DNA synthesis by <em>Escherichia coli</em> DNA polymerase I, allowing the selective labelling of one strand of the DNA duplex.In buffers of low molarity and high pH (8.5), <em>Eco</em>RI cleaves sequences with the form <figure class=/></figure> (Polisky <em>et al.</em>, 1975). Thus it seems that under both sets of conditions the enzyme recognises the four-base-pair core sequence <figure class=/></figure> and that its ability to cleave different adjacent phosphodiester bonds varies with pH and ionic strength.</td> </tr> <tr> <td align=
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