改良大鼠子宫内膜异位症模型的建立及微血管密度观察

目的对皮下筋膜层与腹壁肌层之间移植自体子宫内膜制作的子宫内膜异位症（endometriosis，EMs）模型进行评估。方法取10只雌性未交配性成熟大鼠，术前雌激素诱导，手术开腹取右侧子宫，将自体子宫内膜种植于双侧皮下筋膜层与腹壁肌层之间，术后第29天取出异位组织，进行组织形态学观察，免疫组织化学染色观察微血管密度（Microvessel density，MVD）。结果异位内膜在腹壁内生长，呈隆起囊状小包块，内有黏液，具有正常子宫内膜基本组织结构。异位内膜中微血管密度较在位内膜和正常子宫内膜高。结论此手术方法建立的大鼠子宫内膜异位症模型异位内膜病理改变与EMs患者类似，可以作为子宫内膜异位研究的动物模型。

Improvement of Establishment of a Rat Model of Endometriosis and Observation of Its Microvessel Density (MVD)

Objective To evaluate the rat model of endometriosis （EMs）, conducted by autotransplantation of its own endometrium between the superficial fascia and muscular layer of the abdominal wall. Methods 10 Female virgin rats in estrus stage were induced by estrogen injection prior to operation. During the laparotomy, the right uterus was taken and its endometrium was implanted into the left and right abdominal wall between superficial fascia and muscular layer. Two cysts were formed and they was taken out at 29 days after implantation, examined by histology and immunohistochemistry. Results The ectopic endometrium growing in the abdominal wall formed a cystic mass with a large cavity containing much mucus. It showed an endometrial structure similar to that of normal endometrium. Micrevessel density （MVD） in the ectopic endometrium was higher in comparison with that of eutopic endometrium and normal endometrium. Conclusion These results suggested that the rat model established by this method is suitable for endometriosis research.