Human ferritin light chain gene sequences mapped to several sorted chromosomes |
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Authors: | R V Lebo Y W Kan Mei-Chi Cheung S K Jain J Drysdale |
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Institution: | (1) Howard Hughes Medical Institute and Department of Medicine, University of California, 94143 San Francisco, CA, USA;(2) Department of Biochemistry and Pharmacology, Tufts Medical School, 02111 Boston, MA, USA |
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Abstract: | Summary The iron storage ferritin light-chain gene exhibits multiple restriction enzyme fragments which have been mapped by analyzing sorted human chromosomes. A dual laser chromosome sorter was used to construct spot-blot filter panels representing 22 chromosome fractions. Hybridization of radiolabeled human ferritin-L gene probe to spot-blot panels revealed the ferritin-L gene on more than one chromosome. Miniaturized restriction enzyme analysis was used to map each of the ferritin-L restriction fragments uniquely to one of three chromosomes. This combination of sorted chromosome analyses provides a rapid method to map homologous DNA sequences located on more than one chromosome. |
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