Intensive drying of grids after lead citrate staining lowers precipitate contamination on the surface of ultrathin sections |
| |
Authors: | Khokhlov S E Nevorotin A I |
| |
Affiliation: | Central Research Laboratory, St. Petersburg State Medical University, St. Petersburg. |
| |
Abstract: | Two techniques are proposed to minimize precipitate contamination of ultrathin sections after lead citrate staining. According to the first one, stained and washed grids are placed, sections downward, on a stack of filter papers. The second one involves a consecutive washing of grids with distilled water, 96% ethanol, and n-hexane. Both techniques are equally efficient; the former being simpler in processing, while the latter is superior in the reproducibility of results. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|