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Intensive drying of grids after lead citrate staining lowers precipitate contamination on the surface of ultrathin sections
Authors:Khokhlov S E  Nevorotin A I
Affiliation:Central Research Laboratory, St. Petersburg State Medical University, St. Petersburg.
Abstract:Two techniques are proposed to minimize precipitate contamination of ultrathin sections after lead citrate staining. According to the first one, stained and washed grids are placed, sections downward, on a stack of filter papers. The second one involves a consecutive washing of grids with distilled water, 96% ethanol, and n-hexane. Both techniques are equally efficient; the former being simpler in processing, while the latter is superior in the reproducibility of results.
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