Enhanced sensitivity of Streptomyces seoulensis to menadione by superfluous lipoamide dehydrogenase

Lipoamide dehydrogenase from Streptomyces seoulensis could facilitate menadione-mediated cytochrome c reduction, which was mostly inhibited by superoxide dismutase, indicating the obvious involvement of superoxide radical anion. In this reaction, the production of superoxide radical anion occurred via a menadione semiquinone radical anion. When exposed to menadione, lipoamide dehydrogenase-overexpressing cells showed a much lower survival rate with a concomitant decrease of intracellular protein thiol than the wild-type strain. These results suggest that lipoamide dehydrogenase is a facilitating agent in the redox cycling of quinone compounds in vivo as well as in vitro and could inevitably increase the potential toxicity of the compounds.