松褐天牛触角感器电镜扫描和触角电位反应

应用扫描电镜对松褐天牛的触角感器进行扫描观察，并测定了触角对松树主要挥发物的电生理反应.松褐天牛触角上共有7种感器，即毛形感器、锥形感器、指形感器、棒形感器、瓶形感器、芽形感器以及刺形感器，以毛形和锥形感器的数量最丰富.其中毛形感器和锥形感器各有3个型，指形感器有2个型.毛Ⅱ、毛Ⅲ型和锥Ⅰ、锥Ⅱ型感器表皮结构具有深的纵刻纹，具有嗅觉感器的典型特征.同时对各种感器的数量、长度及分布特点等作了描述.电生理研究结果表明，几种供试化合物及其混合物均能引起松褐天牛雌、雄成虫较强的电生理反应；松褐天牛触角不同部位的EAG反应存在显著差异；剂量试验表明，松褐天牛成虫触角对不同浓度的α-Pinene的EAG反应存在一定的规律性.

Scanning electron microscopic observations of Monochamus alternatus antennal sensilla and their electroantennographic responses

With scanning electron microscope (SEM), this paper observed the shape, category, amount and distribution of the main antenna sensilla of adult Monochamus alternatus, and tested their electroantennographic (EAG) responses to the main volatiles of Pinus spp.. There were seven types of antennal sensilla, i. e., sensilla trichoid, sensilla basiconica, sensilla digit-like, sensilla rod-like, sensilla bottle-like, sensilla bud-like and sensilla chaetica, among which, sensilla trichoid and sensilla basiconica were the most abundant on the antenna surface, and each of them could be divided into three subtypes. Two subtypes of sensilla digit-like could also be observed. The II and III subtypes of sensilla trichoid and I and II subtypes of sensilla basiconica had deep longitudinal grooves on their surface, the typical characteristics of olfactory receptor. The comparison of the EAG response of different parts of Monochamus alternatus antennae to alpha-Pinene showed that each volatile and their compounds could provoke significant EAG responses of both females and males. The dose-response test showed that there was a certain rule in the EAG responses of M. alternatus.