Chemiluminescent acridinium-9-carboxamide boronic acid probes: application to a homogeneous glycated hemoglobin assay |
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Authors: | Adamczyk Maciej Chen Yon-Yih Johnson Donald D Mattingly Phillip G Moore Jeffrey A Pan You Reddy Rajarathnam E |
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Institution: | Abbott Laboratories, Diagnostics Division, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamcyzk@abbott.com |
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Abstract: | Chemiluminescent acridinium-9-carboxamide probes containing 1, 3, 9, and 27 phenylboronic acids were prepared and their chemiluminescent properties evaluated. The relative chemiluminescent signal from the probes varied from 4 to 0.83 x 10(19)counts/mol across the series, while the apparent affinity of the probes for the diabetes marker glycated hemoglobin increased from 211 to 0.43 microM. The dose-dependent modulation of the chemiluminescent intensity of the probes upon binding was used to demonstrate a homogeneous assay for glycated hemoglobin. |
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