Evidence for GC preference by monocot Dicer-like proteins |
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Authors: | Ho Thien Rusholme Pilcher Rachel L Edwards Mary-Lou Cooper Ian Dalmay Tamas Wang Hui |
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Institution: | a NERC/Centre for Ecology and Hydrology (CEH) Oxford, Mansfield Road, Oxford OX1 3SR, UK b Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK c School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK |
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Abstract: | Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production. |
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Keywords: | siRNA Dicer GC content Potyvirus Monocot |
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