Comparative X-ray structure analysis of human and Escherichia coli tRNA(Gly) acceptor stem microhelices |
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| Authors: | Förster C Zerressen-Harte A Fürste J P Perbandt M Betzel Ch Erdmann V A |
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| Institution: | a Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany
b Institute of Biochemistry and Molecularbiology, University of Hamburg, c/o DESY, Notkestr. 85, Building 22a, 22603 Hamburg, Germany |
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| Abstract: | tRNA identity elements assure the correct aminoacylation of tRNAs by the aminoacyl-tRNA synthetases with the cognate amino acid. The tRNAGly/glycyl-tRNA sythetase system is member of the so-called ‘class II system’ in which the tRNA determinants consist of rather simple elements. These are mostly located in the tRNA acceptor stem and in the glycine case additionally the discriminator base at position 73 is required. Within the glycine-tRNA synthetases, the archaebacterial/human and the eubacterial sytems differ with respect to their protein structures and the required tRNA identity elements, suggesting a unique evolutionary divergence.In this study, we present a comparison between the crystal structures of the eubacterial Escherichia coli and the human tRNAGly acceptor stem microhelices and their surrounding hydration patterns. |
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| Keywords: | E coli and human tRNAGly acceptor stem microhelix tRNA identity elements Glycyl-tRNA sythetase (GlyRS)/tRNAGly system Crystal structures RNA hydration Superposition |
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