Crystal structure of a human tRNA(Gly) microhelix at 1.2A resolution |
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| Authors: | Förster C Mankowska M Fürste J P Perbandt M Betzel Ch Erdmann V A |
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| Affiliation: | a Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany
b Institute of Biochemistry and Molecular biology, University of Hamburg, c/o DESY, Notkestr. 85, Building 22a, 22603 Hamburg, Germany |
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| Abstract: | The tRNAGly/glycyl-tRNA synthetase (GlyRS) system belongs to the so-called ‘class II aminoacyl-tRNA synthetase system’ in which tRNA identity elements are assured by rather few and simple determinants mostly located in the tRNA acceptor stem. Regarding evolutionary aspects, the tRNAGly/GlyRS system is a special case. There exist two different types of GlyRS, namely an archaebacterial/human type and a eubacterial type reflecting an evolutionary divergence within this system.Here we report the crystal structure of a human tRNAGly acceptor stem microhelix at 1.2 Å resolution. The local geometric parameters of the microhelix and the water network surrounding the RNA are presented. The structure complements the previously published Escherichia coli tRNAGly aminoacyl stem structure. |
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| Keywords: | Human tRNAGly acceptor stem microhelix Crystal structure tRNA identity elements Glycyl-tRNA synthetase (GlyRS) RNA hydration |
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