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Characterisation of human dental stem cells and buccal mucosa fibroblasts |
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| Authors: | Lindroos Bettina Mäenpää Katja Ylikomi Timo Oja Hannu Suuronen Riitta Miettinen Susanna |
| Affiliation: | a REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Biokatu 12, 33520 Tampere, Finland b Cell Research Center, Medical School, University of Tampere, Tampere, Finland c Department of Clinical Chemistry, University Hospital, Tampere, Finland d Tampere School of Public Health, University of Tampere, Tampere, Finland e Department of Eye, Ear and Oral Diseases, Tampere University Hospital, Finland f Department of Biomedical Engineering, Tampere University of Technology, Finland |
| Abstract: | Human craniofacial stem cells are recently discovered sources of putative mesenchymal stem cells that hold great promise for autogenic or allogenic cell therapy and tissue engineering. Prior to employing these cells in clinical applications, they must be thoroughly investigated and characterized. In this study, the surface marker expression was investigated on dental pulp stem cells (DPSCs), dental follicle cells (DFCs), periodontal ligament stem cells (PDLSCs), and buccal mucosa fibroblasts (BMFs) utilising surface markers for flow cytometry. The osteogenic potential was also examined by bone-associated markers alkaline phosphatase, Runx2, collagen type I, osteocalcin, and osteopontin. The results from our study demonstrate that the dental cell sources exhibit comparable surface marker and bone-associated marker profiles parallel to those of other mesenchymal stem cell sources, yet distinct from the buccal mucosa fibroblasts. Our data support evidence towards clinical applicability of dental stem cells in hard tissue regeneration. |
| Keywords: | FACS Dental pulp Dental follicle Periodontal ligament Buccal mucosa fibroblast Osteogenesis Adipogenesis Chondrogenesis |
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