Protein kinase C increases the activity of the polyoma virus middle T antigen-associated phosphatidylinositol kinase |
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Authors: | L Raptis J Bell J F Whitfield |
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Affiliation: | Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. |
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Abstract: | Exposure of polyoma virus-transformed fibroblasts to the protein kinase C-stimulating phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) is known to increase the transforming potential of the virus's middle T antigen. Here it is shown that this TPA treatment also stimulates an 85 kDa phosphatidylinositol kinase associated with the middle T antigen. Since activation of this kinase is known to be necessary, although not by itself sufficient for the transformation of cells by polyoma virus, bursts of protein kinase C activity, triggered by TPA or various cellular receptors, might enhance the oncogenicity of polyoma virus by stimulating this middle T antigen-associated phosphatidylinositol kinase. |
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